Table I.
Contents of Glc, Fru, and Suc (μg mg−1 cotyledon) detected by high performance anion-exchange chromatography in alcohol extracts of detached and attached cotyledons of seedlings of H. courbaril
| Days | Treatments
|
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Control Seedling
|
Seedling in Darkness
|
Shoot Excised 34 d
|
Detached 19 d Water
|
Detached 34 d Water
|
Detached 41 d Water
|
Detached 41 d 2,4-D
|
|||||||||||||||
| Glc | Fru | Suc | Glc | Fru | Suc | Glc | Fru | Suc | Glc | Fru | Suc | Glc | Fru | Suc | Glc | Fru | Suc | Glc | Fru | Suc | |
| μg mg cot−1 | |||||||||||||||||||||
| 7 | 0.9 | 1.3 | 5.3 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 19 | 0.5 | 1.7 | 5.7 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 26 | 0.4 | 1.8 | 0.6 | 0.3 | 1.4 | 0.9 | – | – | – | 0.4 | 1.7 | 8.2 | – | – | – | – | – | – | – | – | – |
| 34 | 1.9 | 5.8 | 0.6 | 0.4 | 3.3 | 3.1 | – | – | – | 0.3 | 1.4 | 7.5 | – | – | – | – | – | – | – | – | – |
| 41 | 5.2 | 10.4 | 3.7 | 2.0 | 6.6 | 3.0 | 3.1 | 5.5 | 4.4 | 0.2 | 0.8 | 4.2 | 1.1 | 5.8 | 24.7 | – | – | – | – | – | – |
| 48 | 22.1 | 23.1 | 6.0 | 1.9 | 4.9 | 4.6 | 4.4 | 8.6 | 1.4 | 0.1 | 0.3 | 0.6 | 1.5 | 7.9 | 22.2 | 4.5 | 20.9 | 41.0 | 18.4 | 32.9 | 33.8 |
| 54 | 16.3 | 13.8 | 5.3 | 2.5 | 7.2 | 7.7 | 2.0 | 7.2 | 2.3 | – | – | – | 0.2 | 0.6 | 0.1 | 8.5 | 31.8 | 3.7 | 0.9 | 5.7 | 0.9 |
| 61 | – | – | – | – | – | – | 4.6 | 9.0 | 4.4 | – | – | – | – | – | – | – | – | – | – | – | – |
| 68 | – | – | – | – | – | – | 6.0 | 12.2 | 4.6 | – | – | – | – | – | – | – | – | – | – | – | – |
The attached cotyledons were from seedlings grown under control conditions, in the darkness, or with shoot excised above the cotyledons insertions at 34 d. The detached cotyledons were isolated at 19, 34, and 41 d after the beginning of seed imbibition and maintained in darkness with water or 2,4-D (10−6 m). All data represent the analyses of the same three samples of cotyledons used for starch determination. −, Not determined.