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Effect of CNP and FSH on cGMP concentration in cultured COCs. (a) COCs were stimulated by simultaneous addition of CNP (100 nM) and FSH (1 IU/ml) in culture medium. (b) COCs were pre-treated with CNP for 1 h before FSH addition at the time 0 h. The significant differences between CNP and CNP + FSH groups within the same interval of culture are shown above the columns.
