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. 2015 Jan 7;13:1. doi: 10.1186/1477-7827-13-1

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© Blaha et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of cGMP analogues on maturation of pig denuded oocytes and COCs. (a) Maturation of denuded oocytes cultured in medium supplemented with various concentrations of 8-Br-cGMP or (b) 8-CPT-cGMP for 44 h. (c) Effect of FSH on maturation of COCs in medium supplemented with 1 mM 8-Br-cGMP or (d) 1 mM 8-CPT-cGMP for 44 h. C, control oocytes or COCs cultured in medium without FSH and cGMP analogues; GV, germinal vesicle; GVBD, germinal vesicle breakdown; MII, metaphase II. Asterisks above the column indicate significant differences from the corresponding value in the control group. *P < 0.05; **P < 0.01; ***P < 0.001. Approximately 75 denuded oocytes or COCs were included in each group.