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. 2015 Jan 7;13:1. doi: 10.1186/1477-7827-13-1

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© Blaha et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of FSH (1 IU/ml), CNP (100 nM) and 8-CPT-cGMP (1 mM) on activation of MAPK3/1 in cultured COCs. (a) The images show a representative result of immunoblotting of phosphorylated MAPK3/1 (top panels) and total MAPK3/1 (bottom panels) in samples of 25 COCs cultured for 4 h. The graphs represent quantification of the activated MAPK3/1 in COCs by densitometry. The results are shown as proportions of the phosphorylated and total MAPK3/1 and expressed in arbitrary units as a fold strength increase over the proportion found in COCs cultured in control medium. The different superscripts above the columns indicate significant differences (P < 0.05). (b) Time course of MAPK3/1 activation in COCs cultured in medium supplemented with FSH or FSH + 8-CPT-cGMP.