Table 2.
The shortest length for a candidate barcode to reach the maximum discrimination success using genetic distance method
| Markers | Maximum discrimination success (%) | Shortest length (bp) | Primer name | Primer sequence 5' to 3' |
|---|---|---|---|---|
| rbcL | 91.67 | 480 | m-rbcLF | ACAAATTGACTTATTATACTCCTGA |
| m-rbcLR | TCGTCTTTGGTAAAATCAAGTCCA | |||
| matK | 62.5 | 90 | m-matKF | CTTCTTGAACGAATCTATTTCTA |
| m-matKR | CCATAAATTAACAAAGTAATATGT | |||
| trnH-psbA | 62.5 | 50 | m-HAF | TAATCTAGAATTTAGCTACTTCTTC |
| m-HAR | CCTTGATCCACTTGGCTACATCC | |||
| rps16 | 83.33 | 280 | m-rps16F | ATAGGAATGAAGGTGCTCTTG |
| m-rps16R | ATCCTTCCAACAAAATGGCAGCA | |||
| ycf1a | 91.67 | 60 | m-ycf1aF | TTATTACCGAGTTGGAACAACA |
| m-ycf1aR | TTGAGTACGCATAGAACCTTTGAT | |||
| ycf1b | 100 | 110 | m-ycf1bF | AAKCAAGAGACAACTTACCTTGA |
| m-ycf1bR | GGATCAGATGCACAAAACCAAGGAA |