Figure 11.

Expression of CpMYB10 target gene mRNAs in 35S-CpMYB10 plants. RNA gel blotting was conducted to measure the amount of RD29A, RD22, COR15A, P5CS1, ADH1, ABI4, AtMYB2, and AtMYC2 mRNA in transgenic Arabidopsis plants. Each lane was loaded with 10 μg of total RNA from 2-week-old plants grown in normal MS medium and transferred to MS plates containing the indicated compound 16 h before collecting tissue. Graphics show the densitometric quantification of shown bands after normalization against loaded RNA according to Image 1.61 software from NIH. Ethidium bromide-stained rRNAs were used as a loading control (bottom section).