Figure 5.

Expression pattern of CpMYB10 in transgenic Arabidopsis. Three-week-old plants of a representative transgenic Arabidopsis line (9.2) carrying a 1.5-kb CpMYB10 promoter-GUS construct were processed for histochemical GUS staining (A–F). β-Glucoronidase activity is visualized by the blue color. A, Unstressed plants. B, Anthers from unstressed plants. C, Plants treated with 100 μm ABA. D, Roots from plants treated with 100 μm ABA. E, Dehydrated plants. F, Roots from dehydrated plants. G, Quantification of β-Glucoronidase activity in mature seeds, and different developmental stages of untreated or 100-μm ABA-treated seedlings for 16 h or seedlings subjected to dehydration. Treated and untreated untransformed plants were included as negative controls. The results are means of GUS activities from three independent experiments with a repetition in each experiment. Specific GUS activities are expressed as pmol of 4-methylunbelliferone per mg of total protein per min.