. 2013 Winter;4(1):7–12.

Table 2.

Effect of different leptin concentrations on sperm viability, DNA damage, and membrane integrity (Mean ± SEM) in fresh, equilibrated and frozen thawed semen

	Leptin concentration (ng mL ^-1 )	Viability (%)	DNA damage (%)	HOST (%)
Fresh
	0	87.00 ± 0.70 ^a	1.80 ± 0.10 ^a	88.80 ± 0.90 ^a
	10	87.80 ± 0.70 ^a	1.70 ± 0.10 ^a	88.10 ± 1.80 ^a
	20	86.90 ± 0.90 ^a	1.80 ± 0.10 ^a	86.10 ± 1.20 ^a
	50	86.80 ± 0.60 ^a	1.80 ± 0.10 ^a	87.50 ± 1.50 ^a
	100	86.40 ± 0.80 ^a	1.80 ± 0.10 ^a	86.20 ± 1.10 ^a
	200	85.50 ± 0.80 ^a	1.90 ± 0.10 ^a	85.20 ± 1.00 ^a
Equilibrated
	0	77.90 ± 1.50 ^b	3.30 ± 0.20 ^ab	77.50 ± 1.30 ^a
	10	85.40 ± 1.30 ^a	3.00 ± 0.20 ^b	81.20 ± 1.00 ^a
	20	76.10 ± 1.60 ^b	3.80 ± 0.30 ^ab	80.50 ± 1.30 ^a
	50	74.60 ± 1.60 ^b	3.00 ± 0.20 ^b	79.10 ± 1.50 ^a
	100	73.50 ± 1.60 ^bc	4.10 ± 0.30 ^a	79.50 ± 1.20 ^a
	200	69.50 ± 1.70 ^c	4.10 ± 0.20 ^a	76.00 ± 1.50 ^a
Frozen thawed
	0	60.80 ± 1.40 ^a	11.00 ± 0.30 ^a	58.40 ± 1.50 ^a
	10	60.50 ± 1.80 ^a	10.50 ± 0.40 ^a	60.50 ± 1.00 ^a
	20	58.70 ± 1.40 ^a	10.90 ± 0.30 ^a	60.20 ± 1.60 ^a
	50	57.30 ± 1.60 ^ab	11.20 ± 0.30 ^a	57.30 ± 1.20 ^a
	100	52.50 ± 1.90 ^bc	11.70 ± 0.20 ^a	58.50 ± 1.10 ^a
	200	50.40 ± 1.80 ^c	11.90 ± 0.30 ^a	56.20 ± 1.30 ^a

^a,b,c

Superscript letters represents a significant difference (p < 0.05) within columns in each tables.