Figure 3. Knockdown efficiency of lentivirus-mediated siRNA targeting NUF2 in pancreatic cancer cells.
RT–PCR analysis of NUF2 knockdown efficiency in PANC-1 cells (A) and Sw1990 cells (B), respectively. β-actin gene was used as an internal gene. Data represent the means±S.D. of three independent experiments. **P<0.01, ***P<0.001, compared with shCon. Western blot analysis of NUF2 knockdown efficiency in PANC-1 cells (C) and Sw1990 cells (D), respectively. GAPDH protein was used as an internal control. Data represent one of the three independent experiments with similar results.