Experiment 1 |
Total period of the experiment was 25 days consisted of pre-exposure period (21 days) and exposure period (4 days) |
Control (non malathion exposed groups) |
Control |
C |
F = 8, M = 0 |
Basal commercial pelleted diet |
0 mg/l (0 ppm) |
96 h |
Genoprotective investigation: five fish from each group were investigated through evaluation of chromosomal aberrations, frequency of micronuclei and DNA fragmentation |
Bee pollen |
Gr1 |
F = 8, M = 0 |
Basal diet with 2.5% bee pollen |
0 mg/l (0 ppm) |
96 h |
Propolis |
Gr2 |
F = 8, M = 0 |
Basal diet with 2.5% Propolis |
0 mg/l (0 ppm) |
96 h |
Exposed (Malathion exposed groups) |
Control |
Gr3 |
F = 8, M = 0 |
Basal commercial pelleted diet |
5 mg/l (5 ppm) |
96 h |
Bee pollen |
Gr4 |
F = 8, M = 0 |
Basal diet with 2.5% bee pollen |
5 mg/l (5 ppm) |
96 h |
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Propolis |
Gr5 |
F = 8, M = 0 |
Basal diet with 2.5% Propolis |
5 mg/l (5 ppm) |
96 h |
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Experiment 2 |
Total period of the experiment was 31 days consisted of pre-exposure period (21 days) and exposure period (10 days) |
Control (non malathion exposed groups) |
Control |
T1 |
F = 10, M = 5 |
Basal commercial pelleted diet during whole exerimental period |
0 mg/l (0 ppm) |
10 days |
1 – Genoprotective investigation: five females from each group were investigated through evaluation of chromosomal aberrations, frequency of micronuclei and DNA fragmentation 2 – semen analysis: five males from T1, T3, T4, T5 groups were used. 3 – hormonal assay: five males and five females of T1, T3, T4, T5 groups were used |
Bee pollen |
T2 |
F = 10, M = 5 |
Basal diet with 2.5% bee pollen during whole experimental period |
0 mg/l (0 ppm) |
10 days |
Exposed (Malathion exposed groups) |
Control |
T3 |
F = 10, M = 5 |
Basal commercial pelleted diet during whole exerimental period |
1 mg/l (1 ppm) |
10 days |
Bee pollen |
T4 |
F = 10, M = 5 |
Basal diet with 2.5% bee pollen during whole experimental period |
1 mg/l (1 ppm) |
10 days |
Pre-supplemented with bee pollen |
T5 |
F = 10, M = 5 |
Basal diet with 2.5% bee pollen during for 21 days before malathion exposure and re-supplementation with basal diet during exposure period (10 days) |
1 mg/l (1 ppm) |
10 days |