Figure 3.

Mean (±se) nicotine (A, μg/g fresh mass) and trypsin proteinase inhibitor activity (B, TrypPI) from 35-d-old N. attenuata plants grown under low nitrogen (LN) and high nitrogen (HN) supply rates. Leaves growing at nodes 0 (systemic) and 2 (local) were analyzed 3 d after leaves at nodes 2 and 3 were either treated with 20 μL of lanolin containing 150 μg MeJA (MeJA), in 20 μL of pure lanolin (LC), or wounded and treated with 40 μL of M. sexta oral secretions (OS), 40 μL of deionized water (W), or were left unwounded and untreated (C). Asterisks indicate significant differences between members of a treatment and control pair (LC versus MeJA; OS versus W; P < 0.05, t test).