Figure 7.

Activation of CAD1-A promoter by constitutive or transient expression of GaWRKY1 in plants. A, GUS staining and RT-RCR analysis of the GUS and GaWRKY1 transcripts in mature leaves of T2 plants of 5-7 (pCAD1-A::GUS) and 36-2 (35S::GaWRKY1) lines and their F₁ plants containing both constructs. B, Quantitative determination of GUS activities in mature leaves of the plants as indicated. PCR was performed by 28 cycles of amplification, and Arabidopsis Ade phosphoribosyl transferase (APRT) transcripts were amplified as an internal control. C, Transient assay of GUS activities in tobacco leaves 2 d after infiltration with Agrobacterium cells harboring pCAD1-A::GUS/35S::GaWRKY1 (1), pCAD1-A::GUS (2), mpCAD1-A::GUS/35S::GaWRKY1 (3), mpCAD1-A::GUS (4), and 35S::GaWRKY1 (5), respectively.