Skip to main content
. 2014 Sep 5;171(23):5295–5312. doi: 10.1111/bph.12836

Table 3.

Comparison of the binding kinetics of DPCPX in the absence or presence of 10 μM PD81,723 or BC-1 determined by radioligand association and dissociation assay or by the competition association assay

kon (M−1·min−1) koff (min−1)
k1a k3b k2a k4b
DPCPX 1.2 ± 0.1 × 108 1.7 ± 0.4 × 108 0.23 ± 0.01 0.26 ± 0.01
+10 μM PD81,723 1.4 ± 0.1 × 108 1.9 ± 0.4 × 108 0.23 ± 0.01 0.30 ± 0.07
+10 μM BC-1 2.8 ± 0.2 × 108 2.7 ± 0.1 × 108 0.080 ± 0.004 0.072 ± 0.005

Values are means ± SEM of three separate experiments each performed in duplicate.

a

kon (k1) and koff (k2) of unlabelled DPCPX in the absence or presence of 10 μM PD81,723 or BC-1 were determined in [3H]-DPCPX (2.6 nM) association and dissociation assay.

b

kon (k3) and koff (k4) of unlabelled CCPA in the absence or presence of 10 μM PD81,723 or BC-1 were determined in [3H]-DPCPX (2.6 nM) competition association assays.