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. 2014 Nov 24;171(23):5417–5430. doi: 10.1111/bph.12857

Figure 5.

Figure 5

DOP receptor induced astrocytic EAAT1 and EAAT2 expression in a dose-dependent manner. (A) Astrocytes were treated with UFP-512 for 12 h. Then total mRNA was extracted for mRNA analysis. Quantitative PCR analysis showing that UFP-512 induced EAAT1 and EAAT2 mRNA expression in a dose-dependent manner with expression reaching peak at 10 μM. (B) Astrocytes were treated with UFP-512 for 24 h. Then total protein was extracted for protein analysis. Western blot showing that UFP-512 induced EAAT1 protein production in a dose-dependent manner with production reaching a peak at 10 μM and also up-regulated EAAT2 production, which reached a plateau at 10 μM and 100 μM. C, non-treatment; U, UFP-512. *P < 0.05; **P < 0.01. Note that UFP-512 induced EAAT1 and EAAT2 mRNA as well as protein expression in a time-dependent manner.