Figure 14.

No appreciable change in p38 activation after the treatment with PK and MAPK inhibitors in the absence of UFP-512 stimulation. Astrocytes were treated with indicated inhibitor for 24 h. Then total protein was extracted for Western blot. C, non-treatment; Cal, 500 nM calphostin C, a PKC inhibitor; FR, 500 nM FR180204, ERK inhibitor; H89, a PKA inhibitor, 10 μM; LY, 10 μM LY294002, a PI3K inhibitor; pp38, phosphorylated p38; SB, 10 μM SB203580, p38 inhibitor; U0126, an MEK inhibitor, 5 μM. Note that the inhibitors had no effect on p38 activation.