Figure 3.
Effect of DMSO vehicle and effect of dye concentration on IC50 values. (A) Potential antagonistic effects of dye concentration on hCx43 uncoupling potency were tested by determining IC50 values (using a 1-in-2 dilution series) of digoxin and carbenoxolone (Carb.) using parachute cells loaded with low (5 min dye loading time) and high (30 min dye loading time) dye concentrations (representative of three separate experiments). (B) Effect of various concentrations of DMSO in comparison to control (Con.) and 200 μM carbenoxolone (Carb.) on dye transfer (representative of three separate experiments; *P < 0.05 vs. Con.).