Figure 5.

FC-99 reduced the TLR3 expression induced by exogenous IFN-α via STAT1. RAW264.7 cells (A) or peritoneal macrophage (B) was pretreated with FC-99 for 2 h before IFN-α (100 U·mL⁻¹) stimulation for another 3 h and TLR3 mRNA levels were tested by Q-PCR (n = 4). (C, D) RAW264.7 cells were treated with IFN-α (100 U·mL⁻¹) for the indicated time with or without pretreatment with FC-99 for 2 h; protein levels of phospho-STAT1, STAT1 phospho-JAK1, JAK1 and GAPDH were tested by Western blot. Histograms show the relative band intensity from three independent experiments. The data are means ± SEM. ***P < 0.001 versus control; ^###P < 0.001 versus IFN-α treated alone.