Figure 4.

LXA₄ inhibits the proliferative activity of ESCs. (A) LXA₄ (100 nmol·L⁻¹) inhibited DNA replication in ESCs compared with controls as determined by the EdU incorporation assay, Original magnifications: ×200. EdU-positive cell counts were analysed by using software of the light microscope (B), *P < 0.05. (C) Cell cycle analysis indicated the increased population of cells in the G₀/G₁ phase and decreased S and G₂/M phase cells in ESCs treated with LXA₄ (100 nmol·L⁻¹). (D) Proliferation index = (S phase + G₂/M phase)/(G₀/G₁ phase + S phase + G₂/M phase). (E) LXA₄ decreased PCNA protein expression in ESCs detected by imunocytochemical staining. Original magnifications: ×100. PCNA-positive cell counts were analysed by using software of the light microscope (F). These experiments were done three times using different batches of cells and data are means ± SEM; *P < 0.05.