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. 2014 Oct 24;171(21):4955–4969. doi: 10.1111/bph.12819

Figure 4.

Figure 4

Activation of OX40 NF-κB reporter cells by OX40L and 4 combined at various concentrations. (A) OX40 sensor cells with NF-κB-induced SEAP were incubated overnight with a combination of both serial diluted OX40L and 4. Results are represented as the absorbance value of collected cell supernatant at the wavelength of 650 nm. Asterisks denote statistically significant differences compared to CVN 0 within each OX40L group (*P < 0.05, ***P < 0.001); double daggers denote ‡‡‡P < 0.001 between the maxima of OX40L alone (OX40L 33.3 nM, CVN 0) versus CVN alone (OX40L 0, CVN 100 µM) (anova with Dunnett's multiple comparison post hoc test). As compared to OX40L (B), 4 (CVN) alone (C) only achieved a maximum activation of the OX40-triggered response that was about 70%, indicating a partial agonist type response in this system.