Figure 6.

Compound 4 inhibits iT_reg generation. (A) Naïve wild-type CD4⁺ T-cells were activated for three days (Dynabeads plus iT_reg polarizing cytokines, IL-2 + TGF-β) by increasing concentrations of 4 (CVN). Agonist OX40 antibody (OX86) and tartrazine (TZ) were added as positive and negative controls respectively. Inhibitory effect on the induction of iT_reg cells were accessed by gating on the live CD4⁺ T-cell population. (B) Summary of all experiments showing average ± SD of three independent replicates. Data were analysed by anova with Dunnett's post hoc test with *P < 0.05 and **P < 0.01 as compared with T_reg polarizing conditions (IL-2 + TGF-β).