Figure 7.
Compound 4 enhances the induction of TH9 cells. (A) Naïve wild-type CD4+ T-cells were activated for three days (Dynabeads plus iTreg polarizing cytokines, IL-4 + TGF-β) by increasing concentrations of 4 (CVN). Agonist OX40 antibody (OX86) and tartrazine (TZ) were added as positive and negative controls, respectively. Stimulatory effects on the induction of TH9 cells were accessed by gating on the live CD4+ T-cell population. (B) Summary of all experiments showing average ± SD. Data were analysed by anova with Dunnett's post hoc test with *P < 0.05 and ***P < 0.001 as compared with TH9 polarizing conditions (IL-4 + TGF-β).