Median inhibitory concentrations (IC₅₀) and their 95% confidence intervals (CI) for compounds 1–4 for different TNFRSF-ligand interactions as indicated

Compound	IC50 (µM)
	OX40–OX40L	RANK–RANKL	4-1BB–4-1BBL	CD40–CD40L	TNFR1–TNF-α	OX40 binding*1983
	Human	Human	Human	Human	Human	Human
1 (DB36)	1.2	10.4	52.9	27.4	43.6	0.4
	[1.0–2.6]	[8.7–12.6]	[39.3–71.1]	[21.8–34.4]	[33.0–57.5]	[0.2–0.7]
2 (DB71)	2.7	5.3	47.1	3.4	97.3	32.0
	[2.2–3.2]	[4.7–6.0]	[34.4–64.6]	[1.9–6.0]	[71.6–132.4]	[23.7–43.1]
3 (DB15)	1.5	2.0	30.2	11.1	38.4	2.0
	[1.2–2.0]	[1.6–2.4]	[25.5–35.7]	[7.4–16.8]	[30.0–49.3]	[1.2–3.2]
4 (CVN)	1.9	9.2	41.4	23.6	48.4	6.6
	[1.5–2.6]	[7.6–11.1]	[33.2–51.6]	[19.1–29.3]	[42.4–55.3]	[4.2–10.5]
TZ	>2000	>2000	>2000	>2000	>2000	>2000
ErB†	1.7	1.7	10.8	3.1	4.6	1.7
	[1.2–2.6]	[1.4–2.2]	[8.7–13.4]	[2.5–3.8]	[3.6–5.7]	[1.3–2.1]

Notation for small-molecule compounds – 1: DB36 (direct black 36); 2: DB71 (direct blue 71); 3: DB15 (direct blue 15); 4: CVN (chlorazol violet N, direct violet 1.

^*IC₅₀ values obtained in the binding partner assay. CI shown in italic font and in square parentheses.

^†Values obtained in Ganesan et al. (2011), except for the last column, which was measured here.

TZ, tartrazine; ErB, erythrosine B.