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. 2015 Jan 14;8:432. doi: 10.3389/fncel.2014.00432

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Copyright © 2015 Shpak, Zylbertal and Wagner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Carbachol blocks DAPs in AOB mitral cells. (A) Mean current-frequency (I-F) curves of the same cells as in Figure 6 before (blue) and after (green) carbachol (1 μM) application to the bath solution. (B) Representative voltage responses to 40 pA square-pulse stimulation of the same cell in control and carbachol conditions. Spikes were cut at 0 mV. (C) Mean (±s.e.m.) DAP integral of all responses of the same cells, plotted as a function of the firing frequencies. Horizontal lines represent the mean using 10 Hz bins. (D) Mean (±s.e.m.) values of the DAP integral of the responses to three current amplitudes representing low (20 pA), moderate (50 pA), and high (80 pA) stimulation levels. A significant difference (^*p < 0.05, paired t-test corrected for multiple comparisons) was found between control and carbachol conditions for all stimulation levels.