Figure 6.

Immunogold localization of putative monosaccharide transporter in the flesh of apple fruit. The protein reacting with anti-VvHT1 serum mainly resides in the plasma membrane of SEs and is also found in the plasma membrane of PCs at the early (A, SEs; B, PCs), middle (C, PCs; D, SEs) and late (E, PCs; F, SEs) stages of fruit development. Note that there are much fewer transporter molecules in the plasma membrane of CCs (D, F). The number of immunogold particles apparently increases from the early to late developmental stages in the plasma membrane of SEs. The immunogold particles were scarcely found in the cytoplasm or vacuole of SE or PC (A–F). G and H, SE-CC complex (G) and PCs (H) in a control without antiserum. No substantial signal was detected. Bars = 1 μm. Abbreviations are as in Figure 2.