Table 3.
Methylome motifs detected within the C. jejuni IA3902ΔluxS genome.
| Motifa | Modification type | # Of motifs detected | # Of motifs in genome | % Motifs detected | Partner motif | R-M enzymesd,e |
|---|---|---|---|---|---|---|
| GAGNNNNNRTGb | m6A | 717 | 718 | 99.86 | CAYNNNNNCTC | CjeIAORF994P |
| CAYNNNNNCTCb | m6A | 716 | 718 | 99.72 | GAGNNNNNRTG | CjeIAORF994P |
| CAAAYG | m6A | 1761 | 1761 | 100 | CjeIAORF654P | |
| TAAYNNNNNTGCc | m6A | 499 | 499 | 100 | GCANNNNNRTTA | M.CjeIAII |
| GCANNNNNRTTAc | m6A | 494 | 499 | 99 | TAAYNNNNNTGC | M.CjeIAII |
| GAAGAA | m6A | 2559 | 2564 | 99.8 | CjeIAORF32P | |
| RAATTY | m6A | 27298 | 27518 | 99.2 | RAATTY | M.CjeIAI |
a
Sequence motifs are listed in the 5 ′ to 3 ′ direction. Underlined bases indicate the methylated base on the sequence. Motifs containing Y have either T or C nucleotide, while motifs with R have an A or G nucleotide.
b,c
Complementary motif sequences.
d
Last column lists assigned restriction-modification (R-M) enzymes predicted to recognize respective motif(s).
e
The motifs and associated MTases are excellent candidates for the respective R-M system types. However, because the designations are not definitive, further tests are required to confirm the functional statuses of the MTases.