Table 2.
Top 12 OTUS detected and abundances in tagged pyrosequencing analysis of DNA extracts from solid substrate electron donor enrichments.
| Primary electron donor | Terminal electron acceptor | Phylum | OUT designation# | Percent abundance of OTU | ||
|---|---|---|---|---|---|---|
| Elemental Sulfur | Nitrate | Original Enrichment | Transfer 1 | Transfer 2 | ||
| Gammaproteobacteria | Gammaproteobacteria | 9 | 15 | 24 | ||
| Gammaproteobacteria | Pseudomonas sp. | 45 | 42 | 21 | ||
| Gammaproteobacteria | Halomonas sp. | 4 | 3 | 15 | ||
| Flavobacteria | Aequorivita | 9 | 13 | 11 | ||
| Alphaproteobacteria | Rhodobacteraceae | 1 | 8 | 7 | ||
| Gammaproteobacteria | Pseudomonadaceae | 6 | 9 | 6 | ||
| Gammaproteobacteria | Pseudoidomarina | 0 | 2 | 2 | ||
| Gammaproteobacteria | Idiomarina | 6 | 1 | 2 | ||
| Alphaproteobacteria | Thalassospira | 0 | 0 | 2 | ||
| Gammaproteobacteria | Pseudomonadaceae | 3 | 2 | 1 | ||
| Gammaproteobacteria | Marinobacter | 0 | 1 | 1 | ||
| Epsilonproteobacteria | Arcobacter | 13 | 1 | 1 | ||
| Elemental Iron | Nitrate | Original Enrichment | NA | NA | ||
| Gammaproteobacteria | Halomonas | 35 | ||||
| Epsilonproteobacteria | Arcobactor | 32 | ||||
| Flavobacteriia | Aequorivita | 10 | ||||
| Unknown | other | 3 | ||||
| Deltaproteobacteria | Pelobacteraceae | 3 | ||||
| Gammaproteobacteria | Pseudoidiomarina | 3 | ||||
| Gammaproteobacteria | Gammproteobacteria | 2 | ||||
| Epsilonproteobacteria | Sulfurimonas | 2 | ||||
| Gammaproteobacteria | Pseudomonas | 1 | ||||
| unknownProteobacteria | Proteobacteria | 1 | ||||
| Gammaproteobacteria | Idiomarina | 1 | ||||
| Gammaproteobacteria | Halomonadaceae | 1 | ||||
| FexSx | Nitrate | Original Enrichment | Transfer 1 | Transfer 2 | ||
| Firmicutes | Bacillus sp. | 37 | 47 | 42 | ||
| Firmicutes | Clostridium sp. | 7 | 4 | 25 | ||
| Firmicutes | Veillonellaceae | 0 | 10 | 7 | ||
| Actinobacteria | Propionibacterium sp. | 1 | 1 | 7 | ||
| Firmicutes | Paenibacillus sp. | 7 | 7 | 4 | ||
| Betaproteobacteria | Rhodocyclaceae | 8 | 0 | 3 | ||
| Firmicutes | Anoxybacillus sp. | 0 | 0 | 2 | ||
| Gammaproteobacteria | Acinetobactor sp. | 4 | 1 | 2 | ||
| Betaproteobacteria | Diaphorobacter sp. | 1 | 2 | 2 | ||
| Firmicutes | Lachnospiraceae | 0 | 0 | 2 | ||
| Epsilonproteobacteria | Sulfurospirillum sp. | 2 | 3 | 1 | ||
| Firmicutes | Staphylococcus sp. | 2 | 3 | 1 | ||
#
OTU designation based on percent similarity to known sequences (determined using Qiime, see Materials and Methods).
NA designates not applicable because transfers were not performed or not sequenced most likely due to inactivity.