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. Author manuscript; available in PMC: 2015 Jan 14.
Published in final edited form as: Neurology. 2003 Nov 25;61(10):1405–1411. doi: 10.1212/01.wnl.0000094357.10782.f9

Figure 4.

Figure 4

Co-localization of anti-human herpesvirus 6 (anti-HHV-6) gp116/54/64 reactivity in glial fibrillary acidic protein (GFAP)–reactive cells using double-immunofluorescence assay on formalin-fixed paraffin-embedded brain tissue. Positive staining for GFAP (green) but not for HHV-6 gp116/54/64 (red) of astrocytic cell line U251 was visualized with both fluorescein isothiocyanate and rhodamine filters (A). Positive staining for HHV-6 gp116/54/64 but not for GFAP of HHV-6B (strain Z29)–infected SupT-1 cell line (B) was seen after simultaneous incubation with both sets of antibodies. Lateral temporal lobe of Patient 2 shows presence of GFAP-reactive cells (green) (C, arrows) and of HHV-6 gp116/54/64-positive cells (red; D, arrows) incubated with both anti-GFAP and anti-gp116/54/64 primary antibodies. Juxtaposition of the combined images shows co-localization of GFAP and HHV-6 gp116 reactivity (yellow) (E, arrows) (20× magnification). All fields were counterstained with 4,6-diamidino-2-phenylindole (blue) to visualize nuclei.