Skip to main content
. 2015 Jan 14;83(2):591–603. doi: 10.1128/IAI.02811-14

FIG 4.

FIG 4

LytA binds the downregulator FH to impair the activation of the alternative pathway. (A) Proportion of bacteria positive for FH for the D39 wild-type strain and the different mutants. (B) Example of a flow cytometry histogram for FH binding for strains with the D39 genetic background. WT, wild type. (C) Proportion of bacteria positive for FH for the S3 lytA mutant strain and the complemented S3C strain (lytA+) belonging to ST23F. (D) Mean fluorescence intensity (MFI) of FH binding on the surface of the S3 lytA strain and the complemented S3C (lytA+) strain. (E) Example of a flow cytometry histogram for FH binding of the ST23F strains. (F) Direct binding of 10 μg/ml of LytA to different concentrations of FH by ELISA. Error bars represent the SDs, and asterisks indicate statistically significant differences compared to the results for the wild-type strain or between the results for different concentrations of FH compared to those in the absence of protein: *, P < 0.05; **, P < 0.01; ***, P < 0.001. P was <0.01 for the comparison of the FH results between the pspC lytA double mutant and the single mutants.