TABLE 1.

Putative MoCo-dependent enzymes in M. tuberculosis and their predicted/demonstrated function

Locus designation	Gene name	Annotation	Form of cofactor requireda	Functional information and expression	Reference(s)
Rv0197		Possible oxidoreductase	bis-MGD	Rv0197 detected in guinea pig lungs at 30 days postinfection	33
Rv1161	narG	Respiratory and assimilatory nitrate reductase (alpha chain)	bis-MGD	Required for the survival of M. tuberculosis under anaerobic conditions in vitro	20
				Required for protection of M. tuberculosis against acid stress during hypoxia	21
				Reduced narGHJI expression in M. tuberculosis and M. africanum clinical isolates is associated with impaired fitness in macrophages	46
				Required for growth of M. tuberculosis in human macrophages	38
				Required for nitrite production and transcriptional reprogramming during growth of M. tuberculosis within human macrophages	39
				ΔnarG mutant of M. tuberculosis is more sensitive to isoniazid in human macrophages and axenic culture	40
				NarG detected in guinea pig lungs at 30 and 90 days postinfection	33
Rv1442	bisC	Probable biotin sulfoxide reductase	bis-MGD
Rv2900c	fdhF	Possible formate dehydrogenase H	bis-MGD	Rv2900c detected in guinea pig lungs at 30 days postinfection	33
Rv0218		Probable conserved transmembrane protein, some similarity with sulfite oxidases	MoCo
Rv0373c		Probable carbon monoxide dehydrogenase (large chain)	MCDb/sulfurated	Implicated in the ability of M. tuberculosis H37Ra and M. bovis BCG to oxidize CO at physiologically relevant concentrations	47, 48
				CDH from Mycobacterium sp. strain JC1 demonstrated NO dehydrogenase activity	49
Rv3151	nuoG	Probable NADH dehydrogenase I (chain g)	Unknown	M. tuberculosis ΔnuoG mutant has a proapoptotic phenotype in macrophages	28, 50
		NADH-ubiquinone oxidoreductase		Repressed by acid shock	51

^aEnzymatic preferences for various cofactor forms were determined by interrogating the dbTEU database (http://gladyshevlab.org/trace_element/).

^bMolybdopterin cytosine dinucleotide.