FIG 3.

Transfer of B cells into IFrag^−/− mice promotes robust hematopoietic progenitor activity in bone marrow during responses to Pneumocystis lung infection. (A) Comparisons of total bone marrow cell numbers of uninfected IFrag^−/− mice (day 0) to those of IFrag^−/− mice in which B cells from wild-type mice had been transferred and mice in which no B cells were transferred on day 16 after Pneumocystis lung infection. Five mice per group were assessed. (B) Comparative analysis of hematopoietic progenitor activity for the granulocytic/monocytic lineage were performed by plating 1 × 10⁵ total bone marrow cells per mouse in hematopoietic colony-forming assays (CFU assays) using reagents from StemCell Technologies. All samples were plated in duplicate. (C) Bone marrow cell differentiations were performed microscopically and by FACS analysis. The relative amount (percentage) of CD11b⁺ Gr-1^hi-expressing neutrophils in the bone marrow of the comparison groups is shown. For statistical analysis of data, a one-way ANOVA was performed followed by a Tukey posthoc test. Values that are significantly different are indicated as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001.