FIG 5.

B cell transfer into IFrag^−/− mice does not significantly change the serum expression profile of proinflammatory mediators associated with induction of bone marrow dysfunctions following Pneumocystis lung infection. (A) Changes of total bone marrow cell numbers in IFrag^−/− mice in which no cells were transferred (untransferred) and IFrag^−/− mice in which B cells were transferred on days 0, 10 and 16 of Pneumocystis lung infection. (B) Comparisons of Pneumocystis lung burdens displayed as nucleus counts in log₁₀ units between untransferred and B cell-transferred IFrag^−/− mice over the 16-day course of infection. (C to E) Serum cytokine concentrations for IL-1β (C), IL-12p70 (D), and IFN-γ (E) found in transferred and B cell-transferred groups over the course of infection displayed in parallel. For statistical analysis of data between the two groups over time in panels A and B, a two-way ANOVA was performed followed by a Bonferroni posthoc test. For statistical analysis of data within one group over time in panels C to E, a one-way ANOVA was performed followed by a Tukey posthoc test. Values that are significantly different are indicated as follows: *, P < 0.05; **, P < 0.01. For panels C to E, the values were compared to the value for day 0.