B cell transfer into IFrag−/− mice is associated with increased serum levels for immune regulatory cytokines in response to Pneumocystis lung infection. (A to C) Comparative analysis of serum levels for interleukin-10 (A), interleukin-27 (B), and interleukin-1 receptor antagonist (C) in untransferred versus B cell-transferred IFrag−/− mice on day 0, 10, and 16 after Pneumocystis lung infection. For statistical analysis of data between the two groups over time, a two-way ANOVA was performed followed by a Bonferroni posthoc test. Values that are significantly different are indicated as follows: *, P < 0.05; ***, P < 0.001; ###, significantly higher in untransferred animals. For panels C to E, the values were compared to the value for day 0.