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. 2015 Jan 14;83(2):482–491. doi: 10.1128/IAI.02463-14

FIG 1.

FIG 1

Subpopulations and phenotype of peripheral blood DC from CWD patients. Whole blood and PBMC from CWD patients before and after treatment and healthy control subjects were used to determine the percentages of M-DC and P-DC subsets within the mononuclear cell (MNC) population and to analyze the maturation status within the subpopulation of M-DC by FACS. (A) DC in whole-blood specimens were defined as Lin-1− HLA-DR+ and subdivided into M-DC and P-DC subsets according to the expression of CD11c and CD123; M-DC1 and M-DC2 were determined in PBMC preparations as CD19− CD14− live cells according to the expression of CD1c and CD141. SSC, side scatter; FSC, forward scatter. (B) Percentages of total DC among all MNC in whole blood. (C) Percentages of M-DC and P-DC in whole blood and M-DC1 and M-DC2 among MNC in PBMC preparations. (D) The portion of CD83-expressing cells and the expression level of CD86 and CCR7, represented by the mean fluorescence intensity (MFI), are indicated for the M-DC population. (B to D) Dot plots show all data points, and the median value is indicated as a horizontal line. P < 0.01 (**) and P < 0.05 (*) according to Mann-Whitney U test.