FIG 7.

Increased PD-L1 expression in MDSCs from PcP mice. MDSCs (MDSCs/PcP) were isolated from PcP mice at 5 weeks post-Pneumocystis infection. Control Gr1BM cells were isolated from uninfected mice immunosuppressed by weekly injection of anti-CD4 (L3T4) antibody. (A) Total RNA was isolated from the cells, and PD-L1 gene expression was determined by real-time RT-PCR. The average PD-L1 expression level in Gr1BM cells was set as 1, and that in MDSCs was compared to it. Data are presented as means ± SD from three independent experiments. (B) The cells were examined by flow cytometry using anti-Gr-1 and anti-PD-L1 antibodies. The result shown is representative of three independent experiments.