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. Author manuscript; available in PMC: 2015 Jan 14.
Published in final edited form as: Leukemia. 2014 Feb 7;28(8):1606–1616. doi: 10.1038/leu.2014.64

Figure 5.

Figure 5

Immunohistochemical validation of NF-kB pathway activation in BPDCN. Immunohistochemical assay reported an evident nucleocytoplasmatic positivity for p50 (c, d), c-Rel (g, h) and RelA (k, l) in CAL-1 cell line and BPDCN patients, as expected upon NF-kB pathway activation (black arrows indicate example of nucleocytoplasmatic positivity). On the contrary, pDCs isolated from blood (a, e, i, m, q) or packed in aggregates in the lymph node (b, f, j, n, r: black arrows indicate positive internal controls for nucleocytoplasmatic positivity) reveal a weak positivity confined to the cytoplasm. The latter finding is observed also at the determination of p52 (o, p) and RelB (s, t), in both BPDCN patients, CAL-1 cell line and normal pDCs. (Olympus BX41 microscope, Olympus CAMEDIA C-7070 camera; original magnification ×400, colors balanced after acquisition with Adobe Photoshop).