Skip to main content
. Author manuscript; available in PMC: 2015 Jan 14.
Published in final edited form as: Oncogene. 2013 Feb 18;33(6):702–712. doi: 10.1038/onc.2013.13

Figure 6.

Figure 6

miR-125b-1 expression inhibits cell invasion, anchorage-independent growth and colony formation by downmodulating the TACSTD2-MAPK pathway. (a–c) Invasion and growth suppression by ectopically expressed sh-TACSTD2 and miR-125b in FaDu cells. Colonies grown from FaDu cells stably transfected with Lenti-empty, Lenti-sh-TACSTD2 or Lenti-miR125b-1 were counted. The experiments were performed three times using triplicate samples, and average scores are indicated with error bars on the histogram (*P < 0.05). (d) Repression of MAPK pathway by overexpressed miR-125b-1. Western blot analysis of FaDu cells with knocked down TACSTD2 or overexpressing miR-125b-1. Western blots of total protein extracted from wild type (−), control (empty), knockdown TACSTD2 cell pools (sh-TACSTD2) or miRNAoverexpressing cell pools (miR-125b-1) were probed for TACSTD2, phospho-ERK1/2 (p-ERK), total ERK1/2 (total-ERK), MYC and ACTIN. (e) Rescue of MAPK pathway by overexpression of TACSTD2. Western blot analysis of FaDu cells with overexpressing miR-125b-1 indicated recovery pERK and MYC by TACSTD2 overexpression. (f) Overexpression of TACSTD2, pERK and MYC in hOMK107 cells transfected with antimiR- 125b. IB, immunoblot.