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. 2015 Jan 14;5:792. doi: 10.3389/fpls.2014.00792

FIGURE 4.

FIGURE 4

Auxin and cytokinin responses in early somatic embryogenesis. (A–D) Auxin response represented by DR5rev:3XVENUS-N7 correlated with WOX5 induction represented by pWOX5::GFP in embryonic calli induced in SEIM for 16 h (A; 88.24%, n = 85), 24 h (B; 83.33%, n = 96), 2 days (C; 87.50%, n = 88) and 3 days (D; 89.66%, n = 87). pWOX5::GFP signals are in green, DR5rev:3XVENUS-N7 fluorescence signals are in red. (E,F) Auxin response represented by DR5rev::GFP correlated with PLT2 induction represented by pPLT2::RFP in embryonic calli induced in SEIM for 3 days (E; 86.73%, n = 98) and 4 days (F; 90.91%, n = 77). DR5rev::GFP fluorescence signals are in green, pPLT2::RFP signals are in red. (G,H) Cytokinin response represented by pARR7::GFP correlated with WUS induction represented by pWUS::DsRed-N7 in embryonic calli induced in SEIM for 2 days (G; 90.43%, n = 94) and 3 days (H; 85.37%, n = 82). pARR7::GFP fluorescence signals are in green, pWUS::DsRed-N7 signals are in red, and chlorophyll autofluorescence is shown in blue. CP, cotyledon primordia; Co, cotyledons. Scale bars = 80 μm.