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Functions of cytokinin signaling on SE induction. (A–D) Phenotypes of SSE induction from 35S::ARR7 (A), 35S::ARR15 (B), ahk2 ahk4 mutant (C), and ahk3 ahk4 mutant (D) calli grown on SEIM for 8 days. (E) WOX5 transcript signals in WT embryonic callus cultured in SEIM for 24 h; 86.15%, n = 35. (F) Dispersed WOX5 transcript signals in ahk3 ahk4 double mutant callus following culture in SEIM for 24 h; 87.30%, n = 33. Scale bars = 1.2 mm (A–D) and 80 μm (E,F).
