Figure 4. PKC⍺ silencing or overexpression modulate the amount of Cyclin B1 in the nucleus at G2/M checkpoint.

(a) PKC⍺ was silenced and, 48 hours later, cells were synchronized using Nocodazole. A Scrambled siRNA was used as control (Scrambled). Then, nuclei and cytoplasms were separated and Cyclin B1 localization was investigated. Cyclin B1 decreases both in the cytoplasms and in the nuclei. (b) PKC⍺ was overexpressed using WT and DN vectors and cells were treated with Nocodazole for 6 hours. An Empty vector (Empty) was used as control. Cyclin B1 increases both in the nuclei and the cytoplasms. (c) Cells were treated as reported in a) and b) and immunocytochemistry was performed to study the localization of Cyclin B1.