Figure 3. miR-9 activation by HPV E6 was independent of p53 activity.

(A) p53 protein expression in NIKS cells expressing HPV E6 or E6 mutant F2V by Western blot. As a positive control, nocodazole (50ng/ml) was added to the medium to upregulate p53 expression in NIKS vector control cells. p53 protein was detected in F2V-expressing cells and vector control cells, but not in E6-expressing cells. The p53 and β-actin blots were exposed for 50 and 4 seconds, respectively. (B) miR-9 was the most upregulated miRNA in F2V-expressing NIKS cells. Three most upregulated miRNAs among all miRNAs included in the analysis, as identified by expression profiling, are presented in the graph.