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. 2014 Dec 4;5(22):11464–11478. doi: 10.18632/oncotarget.2562

Figure 3. trT2-50 inhibits angiogenin and VEGF-induced HUVEC tube formation.

Figure 3

Freshly isolated HUVECs were plated in a 96-well plate previously coated with Matrigel. Cells were treated with either trT2-50 (2μM) (B and D) or PBS (control) (A and C), in addition to angiogenin (A and B) or VEGF (C and D) (1 μg/ml each). After 8 h of incubation, at 37°C, the plates were photographed. Marked areas are digitally focused by 2-fold (A1-D1). Tube formation was assessed using Image J and the results are represented as percent of control (E). trT2-50 had a significant inhibitory effect on tube formation (*P < 0.01).