Table 3. The 10 probe sets (9 genes) with the lowest coefficient of variation (CV) across all four timepoints (presurgery and 10, 20, and 45 minutes after resection) in normal colon tissue are shown above the dotted line, and 20 well-known housekeeping genes (HKG) with their CV and their rank when sorted for CV are shown below the dotted line.

			Normal tissue		Tumor tissue
Probe ID	Gene	Protein and function	CV	Rank	Rank
1558623_at	LOC729121	Hypothetical LOC729121	0.0000651769	1	22,547
227472_at	DDA1	DET1 and DDB1 associated 1; may be involved in ubiquitination and subsequent proteosomal degradation	0.0000763301	2	5,095
213477_x_at	EEF1A1	Eukaryotic translation elongation factor 1 alpha 1; involved in protein biosynthesis	0.000120035	3	70
206559_x_at			0.00021194	10	464
203172_at	FXR2	Fragile X mental retardation, autosomal homolog 2; RNA-binding protein	0.00012495	4	5,833
202652_at	APBB1	Amyloid beta (A4) precursor protein-binding, family B, member 1; transcription coregulator	0.000140598	5	22,323
209394_at	ASMTL	Acetylserotonin O-methyltransferase-like; unknown function	0.000153558	6	28,176
234891_at	DKFZP547L112	Hypothetical protein DKFZp547L112	0.000198356	7	17,884
212986_s_at	TLK2	Tousled-like kinase 2; involved in chromatin assembly	0.000208644	8	2,237
223148_at	PIGS	Phosphatidylinositol glycan anchor biosynthesis, class S; component of the GPI transamidase complex	0.000210952	9	24,752
210646_x_at	RPL13A	Ribosomal protein L13a, HKG	0.000370481	53	752
201049_s_at	RPS18	Ribosomal protein S18, HKG	0.000581215	185	2,319
230125_at	GUSB	Glucuronidase, beta, HKG	0.000939722	647	19,340
AFFX-HSAC07/X00351_3_at	ACTB	Actin, beta, HKG	0.001080551	941	7,550
216457_s_at	SF3A1	Splicing factor 3a, subunit 1, 120 kDa, HKG	0.001542067	2,363	890
212581_x_at	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase, HKG	0.001735025	3,208	332
201093_x_at	SDHA	Succinate dehydrogenase complex, subunit A, flavoprotein (Fp), HKG	0.002533378	8,062	42,630
203135_at	TBP	TATA box binding protein, HKG	0.003036083	12,044	11,750
203040_s_at	HMBS	Hydroxymethylbilane synthase, HKG	0.003145361	12,988	6,489
1565446_at	HPRT1	Hypoxanthine phosphoribosyltransferase 1, HKG	0.003323065	14,616	23,933
229165_at	MRPL12	Mitochondrial ribosomal protein L12, HKG	0.003428532	15,543	12,742
224695_at	C2orf29	Chromosome 2 open reading frame 29, HKG	0.00344027	15,651	6,081
211296_x_at	UBC	Ubiquitin C, HKG	0.003508935	16,259	2,040
235741_at	PPIA	Peptidylprolyl isomerase A (cyclophilin A), HKG	0.003666547	17,628	33,077
221842_s_at	ZNF131	Zinc finger protein 131, HKG	0.003960494	20,273	20,072
227708_at	EEF1A1	Eukaryotic translation elongation factor 1 alpha 1, HKG	0.004407467	24,160	24,765
1566191_at	SUZ12	Suppressor of zeste 12 homolog (Drosophila), HKG	0.005051028	29,227	49,826
240686_x_at	TFRC	Transferrin receptor (p90, CD71), HKG	0.006000472	35,483	30,303
232311_at	B2M	Beta-2-microglobulin, HKG	0.012872315	49,756	44,528
214023_x_at	TUBB2B	Tubulin, beta 2B, HKG	0.019826709	52,506	53,778