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. 2014 Oct 21;5(22):11709–11722. doi: 10.18632/oncotarget.2606

FIGURE 5. Metformin affects Rac1, Cdc42, and RhoA activation in tumors.

FIGURE 5

(A and B) Western blots of affinity-precipitated (GST-PBD and GST-RBD) tumor lysates from metformin-treated or untreated xenograft mice. GST-PBD was used to precipitate active form of Rac1 and Cdc42 while GST-RBD was used to precipitate active form of RhoA. As a control, pull-down assay by GST alone was carried out. Corresponding bar diagrams showing the activation of these GTPases as calculated by densitometric scanning of signals described in Methods section. Results are average from eight tumors from control and experiment mice. *p < 0.05 vs control.