Figure 3. AUY922 reduced KIT expression and induced apoptosis in GIST430 cells.

GIST430 cells were treated with AUY922 at the indicated doses and times (A) and analyzed by immunoblotting against phospho- and total KIT, MAPK1/3, AKT, or HSPA1A proteins. GIST430 cells were incubated with AUY922 at the indicated doses and IC₅₀ was determined by the cell viability assay (B) or clonogenic assay (C). Cells were treated with 0.1 μM AUY922 for 12 to 48 h and then analyzed by Annexin V staining (D) or immunoblotting against PARP1 (E). All experiments were repeated at least three times. The data are expressed as the mean ± S.E. of two or more independent experiments.