Figure 5. The effect of AUY922 either alone or in a combination with rapamycin in the GIST430 xenograft animal model.

The GIST430 xenograft was established as described in Materials and Methods. Mice received an intraperitoneal injection of DMSO, AUY922 25 mg/kg twice weekly, AUY922 12.5 mg/kg twice weekly, rapamycin 3 mg/kg twice weekly, or a combination of AUY922 and rapamycin for 4 weeks. (A) The tumor volume was determined as 1/2 x length (mm) x width (mm)². The dissected tumors were collected at the end of drug administration (B) and then analyzed by H&E staining (D) and immunohistochemical staining against KIT, MAP1LC3B, HIF1A, or PECAM1 (E). The necrotic areas of the tumor specimens were determined by quantification, as shown in (C). The data are expressed as the mean ± S.D. of two or more independent experiments. * p value is less than 0.05.