Fig. 8. Molecular characterization of CLIC1 activity modulation by metformin.
A) Outside-out single channel experiments in native CHO cells show that anti-FLAG antibody does not inhibit CLIC1 current, differently from metformin (10mM) that closes the channel (single wt channel in control conditions Popen= 0.2±0.01, n=3 total of 6 minutes continuous recording). B) CLIC1 R29A, transfected in CHO cells, is insensitive to metformin, but it is promptly blocked by anti-FLAG antibody (single R29A channel in control conditions Popen=0.21±0.008, n=3 total of 7 minutes continuous recording). C) Inside-out single channel experiments show that metformin fails to inhibit CLIC1 channel opening after more than 30 seconds of continuous recording (single wt channel in control conditions Popen= 0.26±0.02, n=3 total of 10 minutes continuous recording).