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. 2014 Oct 18;5(22):11641–11652. doi: 10.18632/oncotarget.2604

Figure 1. Effect of PEITC on MPM cell lines.

Figure 1

Three cell lines of MPM (Meso4, 11 and 152) were treated with increasing doses of PEITC alone or combined to NAC (5mM) for 72h. Cell viability was determined using Uptiblue reagent. Values represent the mean ± SEM of three independent measurements. B and C, MPM cell lines were treated with three doses of PEITC alone or combined to NAC (5mM) for 24h. Cell death (B) was measured by flow cytometry, after Annexin-V-APC cell staining. Cell death induction is expressed in percentage of annexin-V-APC labeled cells. ROS detection (C) was performed with flow cytometry thanks to a specific molecular probe CM-H2DCFA. Fluorescence values are expressed in Relative Mean Fluorescence Intensity (RMFI). Values represent the mean ± SEM of three independent measurements on three distinct cell lines.