Figure 4. Induction of Lgl3SA reduces invasion in vivo.

(A) Representative images from mice on regular chow or doxycycline chow, showing invasion into the non-injected hemisphere, primarily along the corpus callosum. The area of analysis is the non-injected hemisphere. Within this area, human cells, identified by STEM121 antibody, are shown pseudocoloured red and negative pixels are pseudocoloured blue. (B) Bar graph showing the extent of invasion (i.e. positive staining for STEM121 in the uninjected hemisphere). Data are from three mice per group, with four sections analyzed per mouse. (C) Bar graph showing total numbers of STEM121 positive pixels in both hemispheres in mice from the regular and doxycycline chow mice. (D) As a control, mice were injected with PriGO8A cells transduced with Tet activator lentiviral vector only. One month later mice were randomized to chow with or without doxycycline. After one more month, mice were analyzed for invasion of PriGO8A cells into the non-injected hemisphere as above. Data are from five mice per group, with three sections analyzed per mouse. (E) Bar graph showing STEM121 positive pixels in both hemispheres in mice from the regular and doxycycline chow mice from the experiment in D. Error bars show the mean ± standard deviation. *indicates a p value < 0.05.