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Preconditioning increases brain-derived neurotrophic factor (BDNF) protein expression in the cornu ammonis 1 (CA1) region of hippocampus. Typical micrographs from the CA1 region of organotypic hippocampal slices with (A, D, and G) NeuN (red, neuronal marker) and (B, E, and H) BDNF (green) depicting increased (C, F, and I) colocalization 48 hours after ischemic preconditioning (IPC) or ΨɛRACK treatment versus sham treatment. Scale bar, 50 μm; n=4.
