Table 1.

Refolding conditions for recombinant WT CES1.

Additive	Reducing agent	Catalytically active CES1
0.2 M trehalose	2 mM β-mercaptoethanol	No
0.2 M sucrose	2 mM β-mercaptoethanol	No
0.2 M sorbitol	2 mM β-mercaptoethanol	No
1% glycerol	2 mM β-mercaptoethanol	Yes
1% glycerol	2 mM DTT	No